Each forms a new bridge (bridge amplification). The result is a cluster of DNA forward and reverse strand clones. This works in three basic steps: amplify, sequence, and analyze. The process begins …

Jul 23, 2025 · Illumina sequencing is based on a technique called bridge amplification, whereby DNA molecules are repeatedly replicated on a glass flow cell containing complementary oligonucleotides.

Illumina sequencing technology leverages clonal array formation and proprietary reversible terminator technology for rapid and accurate large-scale sequencing. The innovative and flexible sequencing …

Nov 2, 2024 · Illumina sequencing also uses bridge amplification. In this process, DNA molecules with ligated adapters are used as templates for repeated amplification on a solid surface like a glass slide.

Through a series of amplification reactions known as bridge amplification [4] (Figure 3A), each fragment forms a cluster of identical molecules called clonal clusters (Figure 3B); therefore, every cluster …

Jul 22, 2020 · How does bridge amplification work in next-generation sequencing (NGS)?

A bridging PCR set is constituted by three rounds of amplification reactions, sequentially performed by IWP, BP plus OWP, and OWP, respectively pairing with three nested sequence-specific primers (SSP).